The Toro-Semliki Wildlife Reserve occupies 200 sq. miles of the relatively flat Semliki River Valley, located just north of the town of Fort Portal, near the eastern edge of the western Great Rift Valley in Uganda, and about an hour northeast of the Sempaya Gate into Semuliki National Park at the border with the Congo.

The southern boundary of the reserve is in the foothills of the Ruwenzori Mountains, but the reserve itself has an elevation of only 3800 feet. The reserve is predominantly savanna grassland, but since 1970 an increasing area of regenerating thicket woodland and woodland savanna has appeared. One of the major features of the reserve is the Wasa River, which passes north through the length of the reserve to empty into Lake Albert. There are a number of other rivers and streams, including the Semliki, all of which empty into Lake Albert and which support a varied and abundant woodland.

Indiana University has conducted an aggressive chimpanzee study in the area since 1998. While neither as habituated or accessible as chimps in nearby Kibale National Park, researchers are quite accommodating and often take guests of the lodge on their field studies. The reserve also supports a variety of other primates including redtail monkeys, blue monkeys, vervets, baboons, and black and white colobus.

The grasslands are dominated by Ugandan kob, impala, buffalo and a variety of smaller duikers. Game viewing at the edge of the grasslands near and along Lake Albert provides bountiful bird life, hippos and elephant.